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大豆蛋白-磷脂酰膽堿納米乳液的顯微觀測及界面結(jié)構(gòu)分析

發(fā)布時間：2019/11/4 15:43:50     瀏覽次數(shù)：1111

大豆蛋白-磷脂酰膽堿納米乳液的顯微觀測及界面結(jié)構(gòu)分析
  
DOI：
中文關鍵詞: 納米乳液  拉曼光譜  顯微共聚焦  超聲  高壓均質(zhì)

英文關鍵詞: nanoemulsion  raman spectroscopy  microscopic confocal  ultrasound  high pressure homogenization

   超聲及高壓均質(zhì)制備的大豆蛋白-磷脂酰膽堿納米乳液球狀形態(tài)，大豆蛋白完全吸附于納米乳液的界面，呈現(xiàn)核殼狀結(jié)構(gòu)。在大豆蛋白-磷脂酰膽堿納米乳液形成中，界面吸附重排作用下，大豆蛋白結(jié)構(gòu)趨向于α-螺旋及無規(guī)卷曲的二級結(jié)構(gòu)組成。超聲制備納米乳液的界面蛋白疏水性基團暴露程度高于高壓均質(zhì)制備的。在高壓均質(zhì)制備納米乳液的界面，磷脂酰膽堿與大豆蛋白表現(xiàn)出較強的疏水交互作用。拉曼顯微觀測證實大豆蛋白-磷脂酰膽堿在納米乳液界面均勻分布，更多的大豆蛋白在超聲納米乳液乳滴界面分布。

    Ultrasonic and high pressure homogenously prepared soybean protein-phosphatidylcholine nanoemulsion spherical morphology， soybean protein completely adsorbed at the interface of nanoemulsion， showing a core-like structure. Soybean protein-phosphatidylcholine nano-emulsion formation in the interface adsorption rearrangement of soybean protein structure tends to α-helix and random curl of the secondary structure， the hydrophobicity of the interface protein of the nanoemulsion was higher than that of the high pressure homogenization， phosphatidylcholine showed a stronger hydrophobic interaction with soybean protein at the interface of high pressure homogenization of nanoemulsion. Raman microscopic observation confirmed the uniform distribution of soybean protein-phosphatidylcholine at the interface of nanoemulsion and the distribution of more soybean protein at the emulsion droplet interface of ultrasonic nanoemulsion.