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乙醇適應對鼠傷寒沙門氏菌及其菌膜耐致死脅迫的影響

發(fā)布時間：2019/11/4 15:55:32     瀏覽次數(shù)：1143

乙醇適應對鼠傷寒沙門氏菌及其菌膜耐致死脅迫的影響
Effects of Ethanol Adaptation on the Resistance to Lethal Stress of Salmonella typhimurium and Its Biofilm
  
DOI：
中文關鍵詞: 鼠傷寒沙門氏菌  菌膜  乙醇適應  蘋果酸

英文關鍵詞: Salmoenlla typhimurium  biofilm  ethanol adaptation  malic acid

本文主要研究鼠傷寒沙門氏菌在玻璃表面生物菌膜（BF）的生長特性和乙醇作用方式對鼠傷寒沙門氏菌菌膜形成的影響以及乙醇適應處理對浮游菌與菌膜耐致死脅迫的作用。采用結晶紫染色觀察鼠傷寒沙門氏菌菌膜的形成，用酶標儀測量波長570 nm處不同條件下生物菌膜的生物量，用平板菌落計數(shù)衡量乙醇適應處理對浮游菌及菌膜耐致死脅迫的影響。結果表明，結晶紫染色可清晰觀察到鼠傷寒沙門氏菌在玻璃表面形成的生物菌膜，菌膜形成的緊密網(wǎng)狀結構隨著時間的延長而增強。乙醇作用方式對菌膜形成有顯著影響，加入不同含量的乙醇后培養(yǎng)48 h，乙醇對菌膜形成有顯著的抑制作用。預先培養(yǎng)菌24 h后加入5%的乙醇，能顯著促進菌膜的生長。菌膜形成過程中，當營養(yǎng)條件為1/10 TSB，5%乙醇適應能增加菌體對蘋果酸的耐受性，增加浮游菌對12%的乙醇及5 mg/mL蘋果酸的耐受性。

  The objectives of this study were to investigate the growth characteristics of Salmonella typhimurium biofilm（BF） on glass surface， effect of ethanol activity on the formation of S. typhimurium BF and effect of ethanol adaptation on the resistance of planktonic and BF to lethal stress. The morphology of S. typhimurium BF on the glass surface was observed by crystal violet staining， BF biomass by Thermo Scientific Microplate Reader at 570 nm and ethanol adaptation by plate colony count. Results showed that S. typhimurium BF formed on the glass surface could be directly observed by crystal violet staining， and reticular structure formed by S. typhimurium BF became denser with incubation time. Ethanol activity had significantly different effects on the formation of BF. Ethanol added at different level during 48 h incubation of S. typhimurium inhibited the formation of BF significantly， however， ethanol added after the incubation of S. typhimurium for 24 h at 5% level only promoted the growth of BF. Ethanol at level of 5% increased the tolerance of bacteria to malic acid in BF formation under the condition of 1/10 TSB. In addition， 5% ethanol adaptation increased planktonic resistance to 12% ethanol and 5 mg/mL malic acid.